Rapid ¹³C Hyperpolarization of the TCA Cycle Intermediate α-Ketoglutarate via SABRE-SHEATH.

ABSTRACT: α-Ketoglutarate is a key biomolecule involved in a number of metabolic pathways─most notably the TCA cycle. Abnormal α-ketoglutarate metabolism has also been linked with cancer. Here, isotopic labeling was employed to synthesize [1-¹³C,5-¹²C,D₄]α-ketoglutarate with the future goal of utilizing its [1-¹³C]-hyperpolarized state for real-time metabolic imaging of α-ketoglutarate analytes and its downstream metabolites in vivo. The signal amplification by reversible exchange in shield enables alignment transfer to heteronuclei (SABRE-SHEATH) hyperpolarization technique was used to create 9.7% [1-¹³C] polarization in 1 minute in this isotopologue. The efficient ¹³C hyperpolarization, which utilizes parahydrogen as the sour

SUBMITTER: Adelabu I 

PROVIDER: S-EPMC9907724 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature