Conformational rearrangements in the sensory RcsF/OMP complex mediate signal transduction across the bacterial cell envelope.

ABSTRACT: Timely detection and repair of envelope damage are paramount for bacterial survival. The Regulator of Capsule Synthesis (Rcs) stress response can transduce the stress signals across the multilayered gram-negative cell envelope to regulate gene expression in the cytoplasm. Previous studies defined the overall pathway, which begins with the sensory lipoprotein RcsF interacting with several outer membrane proteins (OMPs). RcsF can also interact with the periplasmic domain of the negative regulator IgaA, derepressing the downstream RcsCDB phosphorelay. However, how the RcsF/IgaA interaction is regulated at the molecular level to activate the signaling in response to stress remains poorly understood. In this study, we used a site-saturated mutant library of rcsF to carry out several independent

SUBMITTER: Lach SR 

PROVIDER: S-EPMC9907809 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature