Epoxidized graphene grid for highly efficient high-resolution cryoEM structural analysis.

ABSTRACT: Functionalization of graphene is one of the most important fundamental technologies in a wide variety of fields including industry and biochemistry. We have successfully achieved a novel oxidative modification of graphene using photoactivated ClO₂^· as a mild oxidant and confirmed the oxidized graphene grid is storable with its functionality for at least three months under N₂ atmosphere. Subsequent chemical functionalization enabled us to develop an epoxidized graphene grid (EG-grid™), which effectively adsorbs protein particles for electron cryomicroscopy (cryoEM) image analysis. The EG-grid dramatically improved the particle density and orientation distribution. The density maps of GroEL and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were reconstruct

SUBMITTER: Fujita J 

PROVIDER: S-EPMC9908306 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature