Gene set enrichment analysis and ingenuity pathway analysis to verify the impact of Wnt signaling in psoriasis treated with Taodan granules.

ABSTRACT:

Background

Taodan granules (TDGs), traditional Chinese herbals, have effectiveness in relieving skin erythema, scales, and other symptoms of psoriasis. Yet mechanisms of TDGs remain indistinct.

Objective

To indicate the molecular mechanisms of TDGs in treating psoriasis.

Materials and methods

Primarily, transcriptional profiling was applied to identify differentially expressed genes (DEGs), proceeding with Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) analysis were used for functional enrichment analysis. Subsequently, levels of selected genes were validated by RT-PCR and western blotting.

Results

The GSEA results revealed TDGs could down-regulate the Wnt

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9908442 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature