Mining and application of constitutive promoters from Rhodosporidium toruloides.

ABSTRACT: Rhodosporidium toruloides is an oleaginous yeast under development with promising industrial applications. Since promoters of different strengths have been demonstrated as an efficient strategy to fine-tune gene expression in synthetic biology, a set of constitutive promoters with strengths varying over 2 orders of magnitude were identified in R. toruloides through transcriptome analysis under different growth conditions. Promoter candidates were first cloned and characterized using an enhanced green fluorescent protein (EGFP) as a reporter under eight conditions, and 31 promoters were identified with strength varied from 0.1 to 19.0 folds of the commonly used strong promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (P_GPD1). The resultant promoters were then used to

SUBMITTER: Guo X 

PROVIDER: S-EPMC9908808 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature