Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells.

ABSTRACT: The effects of hydrogen peroxide (H₂O₂) on the osteogenic differentiation of primary chicken mesenchymal stem cells (MSCs) were investigated. MSCs were subjected to an osteogenic program and exposed to various concentrations of H₂O₂ for 14 days. Results showed that high concentrations of H₂O₂ (200 and 400 nM) significantly increased pro-apoptotic marker CASP8 expression and impaired osteogenic differentiation, as indicated by decreased mRNA expression levels of osteogenesis-related genes and reduced in vitro mineralization. In contrast, long-term H₂O₂ exposure promoted basal expression of adipogenic markers at the expense of osteogenesis in MSCs during osteogenic differentiation, and incre

SUBMITTER: Tompkins YH 

PROVIDER: S-EPMC9909420 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature