In-Vial Detection of Protein Denaturation Using Intrinsic Fluorescence Anisotropy.

ABSTRACT: The conventional quality control techniques for identifying the denaturation of biopharmaceuticals includes sodium dodecyl sulfate-polyacrylamide gel electrophoresis for identifying fragmentation, ion exchange chromatography and isoelectric focusing for identifying deamidation, reverse-phase high-performance liquid chromatography (HPLC) for identifying oxidation, and size-exclusion HPLC for identifying aggregation. These stability assessments require essential processes that are destructive to the product tested. All these techniques are lab based and require sample removal from a sealed storage vial, which can breach the sterility. In this work, we investigate the heat- and surfactant-induced denaturation of an in-vial-stored model protein, bovine serum albumin (BSA), by analyzing its int

SUBMITTER: Chullipalliyalil K 

PROVIDER: S-EPMC9909669 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature