In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract.

ABSTRACT: Standard cell culture practices require the addition of animal-derived serum to culture media to achieve adequate cell growth. Typically, 5-10% by volume of fetal bovine serum (FBS) is used, which accounts for a vast majority of the media cost while also imposing environmental and ethical concerns associated with the use of animal serum. Here we tested the efficacy of culturing cells by replacing serum in the media with algae extract and select additives. Using LC-MS, we compared molecular signatures of FBS to Chlorella algae extracts and identified NAD(H)/NADP(H) as common and relatively abundant features in their characteristic profiles. Bovine fibroblasts, cultured in serum-free media supplemented with C. vulgaris extract and just two growth factors plus insulin, showed significant grow

SUBMITTER: Defendi-Cho G 

PROVIDER: S-EPMC9909908 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature