Ultra-sensitive isotope probing to quantify activity and substrate assimilation in microbiomes.

ABSTRACT:

Background

Stable isotope probing (SIP) approaches are a critical tool in microbiome research to determine associations between species and substrates, as well as the activity of species. The application of these approaches ranges from studying microbial communities important for global biogeochemical cycling to host-microbiota interactions in the intestinal tract. Current SIP approaches, such as DNA-SIP or nanoSIMS allow to analyze incorporation of stable isotopes with high coverage of taxa in a community and at the single cell level, respectively, however they are limited in terms of sensitivity, resolution or throughput.

Results

Here, we present an ultra-sensitive, high-throughput protein-based stable isotope probing approach (Protein-SIP), which cuts cost for labeled sub

SUBMITTER: Kleiner M 

PROVIDER: S-EPMC9909930 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature