Single cell cortical bone transcriptomics define novel osteolineage gene sets altered in chronic kidney disease.

ABSTRACT:

Introduction

Due to a lack of spatial-temporal resolution at the single cell level, the etiologies of the bone dysfunction caused by diseases such as normal aging, osteoporosis, and the metabolic bone disease associated with chronic kidney disease (CKD) remain largely unknown.

Methods

To this end, flow cytometry and scRNAseq were performed on long bone cells from Sost-cre/Ai9⁺ mice, and pure osteolineage transcriptomes were identified, including novel osteocyte-specific gene sets.

Results

Clustering analysis isolated osteoblast precursors that expressed Tnc, Mmp13, and Spp1, and a mature osteoblast population defined by Smpd3, Col1a1, and Col11a1. Osteocytes were demarcated by Cd109, Ptprz1, Ramp1,

SUBMITTER: Agoro R 

PROVIDER: S-EPMC9910177 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature