Non-B-form DNA tends to form in centromeric regions and has undergone changes in polyploid oat subgenomes.

ABSTRACT: Centromeres are the specialized regions of the chromosomes that direct faithful chromosome segregation during cell division. Despite their functional conservation, centromeres display features of rapidly evolving DNA and wide evolutionary diversity in size and organization. Previous work found that the noncanonical B-form DNA structures are abundant in the centromeres of several eukaryotic species with a possible implication for centromere specification. Thus far, systematic studies into the organization and function of non-B-form DNA in plants remain scarce. Here, we applied the oat system to investigate the role of non-B-form DNA in centromeres. We conducted chromatin immunoprecipitation sequencing using an antibody to the centromere-specific histone H3 variant (CENH3); this accurately p

SUBMITTER: Liu Q 

PROVIDER: S-EPMC9910436 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature