Piezo mechanosensory channels regulate centrosome integrity and mitotic entry.

ABSTRACT: Piezo1 and 2 are evolutionarily conserved mechanosensory cation channels known to function on the cell surface by responding to external pressure and transducing a mechanically activated Ca²⁺ current. Here we show that both Piezo1 and 2 also exhibit concentrated intracellular localization at centrosomes. Both Piezo1 and 2 loss-of-function and Piezo1 activation by the small molecule Yoda1 result in supernumerary centrosomes, premature centriole disengagement, multi-polar spindles, and mitotic delay. By using a GFP, Calmodulin and M13 Protein fusion (GCaMP) Ca²⁺-sensitive reporter, we show that perturbations in Piezo modulate Ca²⁺ flux at centrosomes. Moreover, the inhibition of Polo-like-kinase 1 eliminates Yoda1-induced centriole disengagement. Because prev

SUBMITTER: David L 

PROVIDER: S-EPMC9910506 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature