Human dermal fibroblast-derived exosomes induce macrophage activation in systemic sclerosis.

ABSTRACT:

Objectives

Prior work demonstrates that co-cultured macrophages and fibroblasts from patients with SSc engage in reciprocal activation. However, the mechanism by which these cell types communicate and contribute to fibrosis and inflammation in SSc is unknown.

Methods

Fibroblasts were isolated from skin biopsies obtained from 7 SSc patients or 6 healthy age and gender-matched control subjects following written informed consent. Human donor-derived macrophages were cultured with exosomes isolated from control or SSc fibroblasts for an additional 48 h. Macrophages were immunophenotyped using flow cytometry, qRT-PCR and multiplex. For mutual activation studies, exosome-activated macrophages were co-cultured with SSc or healthy fibroblasts using Transwells.

Results

Macroph

SUBMITTER: Bhandari R 

PROVIDER: S-EPMC9910573 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature