Discovery of antibodies and cognate surface targets for ovarian cancer by surface profiling.

ABSTRACT: Although antibodies targeting specific tumor-expressed antigens are the standard of care for some cancers, the identification of cancer-specific targets amenable to antibody binding has remained a bottleneck in development of new therapeutics. To overcome this challenge, we developed a high-throughput platform that allows for the unbiased, simultaneous discovery of antibodies and targets based on phenotypic binding profiles. Applying this platform to ovarian cancer, we identified a wide diversity of cancer targets including receptor tyrosine kinases, adhesion and migration proteins, proteases and proteins regulating angiogenesis in a single round of screening using genomics, flow cytometry, and mass spectrometry. In particular, we identified BCAM as a promising candidate for targeted thera

SUBMITTER: Schrofelbauer B 

PROVIDER: S-EPMC9910589 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature