Silencing MYOT Expression May Inhibit Autophagy in Human Skeletal Muscle Cells.

ABSTRACT: Muscle diseases are closely related to autophagy disorders. Studies of autophagy inhibition indicated the importance of autophagy in muscle regeneration, while activation of autophagy can restore muscle function in some myopathies. Previous studies have revealed that mutations in the MYOT gene may lead to several kinds of hereditary myopathies. However, whether the autophagy played a crucial role in hereditary myopathy caused by MYOT mutations was still not clear. In this study, we established the MYOT knockdown human skeletal muscle cell models (HSkMCs) by small interfering RNA. Real-time PCR and Western blot studies found that the expression of p62 and LC3B-II was decreased dramatically, which suggested that silencing MYOT expression may regulate

SUBMITTER: Lin ZJ 

PROVIDER: S-EPMC9911237 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature