HyU: Hybrid Unmixing for longitudinal in vivo imaging of low signal-to-noise fluorescence.

ABSTRACT: The expansion of fluorescence bioimaging toward more complex systems and geometries requires analytical tools capable of spanning widely varying timescales and length scales, cleanly separating multiple fluorescent labels and distinguishing these labels from background autofluorescence. Here we meet these challenging objectives for multispectral fluorescence microscopy, combining hyperspectral phasors and linear unmixing to create Hybrid Unmixing (HyU). HyU is efficient and robust, capable of quantitative signal separation even at low illumination levels. In dynamic imaging of developing zebrafish embryos and in mouse tissue, HyU was able to cleanly and efficiently unmix multiple fluorescent labels, even in demanding volumetric timelapse imaging settings. HyU permits high dynamic range ima

SUBMITTER: Chiang HJ 

PROVIDER: S-EPMC9911352 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature