Parasite detection and quantification in avian blood is dependent on storage medium and duration.

ABSTRACT: Studies of parasites in wild animal populations often rely on molecular methods to both detect and quantify infections. However, method accuracy is likely to be influenced by the sampling approach taken prior to nucleic acid extraction. Avian Haemosporidia are studied primarily through the screening of host blood, and a range of storage mediums are available for the short- to long-term preservation of samples. Previous research has suggested that storage medium choice may impact the accuracy of PCR-based parasite detection, however, this relationship has never been explicitly tested and may be exacerbated by the duration of sample storage. These considerations could also be especially critical for sensitive molecular methods used to quantify infection (qPCR). To test the effect of storage

SUBMITTER: Lynton-Jenkins JG 

PROVIDER: S-EPMC9911630 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature