An optimized proteomics-based approach to estimate blood contamination and cellular heterogeneity of frozen placental tissue.

ABSTRACT:

Introduction

Archived human placental tissue specimens are vital for studying placenta pathophysiology and toxicology. Proteomics analysis of placental tissue provides mechanistic and translational information, but the highly perfused and heterogenous nature of the placenta creates confounding technical variability. In this study, we developed an optimized proteomics-based approach to address the technical variability of proteomics data by normalizing blood contamination and cellular heterogeneity of archived placenta samples.

Methods

Placenta samples (n = 99) were homogenized, digested using trypsin, and analyzed by liquid chromatography mass-spectrometry. Label-free quantification (LFQ) intensities of the proteins were analyzed for their correlation with blood (albumin) an

SUBMITTER: Kruger L 

PROVIDER: S-EPMC9912121 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature