ABCB5⁺ mesenchymal stromal cells therapy protects from hypoxia by restoring Ca²⁺ homeostasis in vitro and in vivo.

ABSTRACT:

Background

Hypoxia in ischemic disease impairs Ca²⁺ homeostasis and may promote angiogenesis. The therapeutic efficacy of mesenchymal stromal cells (MSCs) in peripheral arterial occlusive disease is well established, yet its influence on cellular Ca²⁺ homeostasis remains to be elucidated. We addressed the influence of ATP-binding cassette subfamily B member 5 positive mesenchymal stromal cells (ABCB5⁺ MSCs) on Ca²⁺ homeostasis in hypoxic human umbilical vein endothelial cells (HUVECs) in vitro and in vivo.

Methods

Hypoxia was induced in HUVECs by Cobalt (II) chloride (CoCl₂) or Deferoxamine (DFO). Dynamic changes in the cytosolic- and endoplasmic reticulum (ER) Ca²⁺ and changes in reactive oxygen species were

SUBMITTER: Yan K 

PROVIDER: S-EPMC9912525 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature