Inhibition of DNA methylation attenuates lung ischemia-reperfusion injury after lung transplantation.

ABSTRACT:

Objective

DNA methylation plays an important role in inflammation and oxidative stress. This study aimed to investigate the effect of inhibiting DNA methylation on lung ischemia-reperfusion injury (LIRI).

Methods

We adopted a completely random design for our study. Thirty-two rats were randomized into the sham, LIRI, azathioprine (AZA), and pluripotin (SC1) groups. The rats in the LIRI, AZA, and SC1 groups received left lung transplantation and intravenous injection of saline, AZA, and SC1, respectively. After 24 hours of reperfusion, histological injury, the arterial oxygen partial pressure to fractional inspired oxygen ratio, the wet/dry weight ratio, protein and cytokine concentrations in lung tissue, and DNA methylation in lung tissue were evaluated. The pulmonary endoth

SUBMITTER: Liu MY 

PROVIDER: S-EPMC9912569 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature