Developing a single-stage continuous process strategy for vitamin B₁₂ production with Propionibacterium freudenreichii.

ABSTRACT:

Background

Vitamin B₁₂ is a widely used compound in the feed and food, healthcare and medical industries that can only be produced by fermentation because of the complexity of its chemical synthesis. Besides, the use of Generally Recognized as Safe (GRAS) and Qualified Presumption of Safety (QPS) microorganisms, like Propionibacterium freudenreichii, especially non-GMO wild-type producers, are becoming an interesting alternative in markets where many final consumers have high health and ecological awareness. In this study, the production of vitamin B₁₂ using the Propionibacterium freudenreichii NBRC 12391 wild-type strain was characterized and optimized in shake flasks before assessing several scale-up strategies.

Results

Initial results established tha

SUBMITTER: Calvillo A 

PROVIDER: S-EPMC9912679 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature