The LH-DH module of bacterial replicative helicases is the common binding site for DciA and other helicase loaders.

ABSTRACT: During the initiation step of bacterial genome replication, replicative helicases depend on specialized proteins for their loading onto oriC. DnaC and DnaI were the first loaders to be characterized. However, most bacteria do not contain any of these genes, which are domesticated phage elements that have replaced the ancestral and unrelated loader gene dciA several times during evolution. To understand how DciA assists the loading of DnaB, the crystal structure of the complex from Vibrio cholerae was determined, in which two VcDciA molecules interact with a dimer of VcDnaB without changing its canonical structure. The data showed that the VcDciA binding site on VcDnaB is the conserved module formed by the linker helix LH of one monomer and the determinant helix DH of the second monomer. In

SUBMITTER: Cargemel C 

PROVIDER: S-EPMC9912922 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature