The imbalance of liver resident macrophages polarization promotes chronic autoimmune hepatitis development in mice.

ABSTRACT:

Background

Autoimmune hepatitis (AIH) is a chronic immune-mediated inflammatory liver disease. At present, it is largely unknown how the innate immune cells influence AIH development.

Objective

To inquiry about mechanism of liver resident macrophages in AIH development, thus offering a new direction for AIH targeted treatment.

Methods

The liver resident macrophages were eliminated by clodronate liposomes in AIH liver tissues, followed by HE and Picrosirius assay to detect liver fibrosis and lymphocyte infiltration. The liver resident macrophages polarization was detected by Immunohistochemistry and qPCR. The collagenase digestion was used to isolate Kupffer cells from AIH mice liver tissues and pro-/anti-inflammatory cytokines were determined by qPCR.

Results

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SUBMITTER: Chi G 

PROVIDER: S-EPMC9912947 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature