Efficient Muscle Regeneration by Human PSC-Derived CD82⁺ ERBB3⁺ NGFR⁺ Skeletal Myogenic Progenitors.

ABSTRACT: Differentiation of pluripotent stem cells (PSCs) is a promising approach to obtaining large quantities of skeletal myogenic progenitors for disease modeling and cell-based therapy. However, generating skeletal myogenic cells with high regenerative potential is still challenging. We recently reported that skeletal myogenic progenitors generated from mouse PSC-derived teratomas possess robust regenerative potency. We have also found that teratomas derived from human PSCs contain a skeletal myogenic population. Here, we showed that these human PSC-derived skeletal myogenic progenitors had exceptional engraftability. A combination of cell surface markers, CD82, ERBB3, and NGFR enabled efficient purification of skeletal myogenic progenitors. These cells expressed PAX7 and were able to different

SUBMITTER: Xie N 

PROVIDER: S-EPMC9913306 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature