Antithymocyte Globulin Inhibits CD8⁺ T Cell Effector Functions via the Paracrine Induction of PDL-1 on Monocytes.

ABSTRACT:

Background

Antithymocyte globulins (ATG) are T cell-depleting antibodies used in solid organ transplantation for induction therapy in sensitized patients with a high risk of graft rejection. Previously described effects besides the depletion of T cells have suggested additional modes of action and identified further cellular targets.

Methods

We examined the transcriptional changes arising in immune cells from human blood after ex vivo stimulation with ATG at the single-cell level to uncover additional mechanisms by which ATG regulates T cell activity and effector functions.

Findings

Analysis of the paracrine factors present in the plasma of ATG-treated whole blood revealed high levels of chemokines and cytokines, including interferon-γ (IFN-γ). Furthermore, we identif

SUBMITTER: Copic D 

PROVIDER: S-EPMC9913606 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature