IQGAP1 Is a Phosphotyrosine-Regulated Scaffold for SH2-Containing Proteins.

ABSTRACT: The scaffold protein IQGAP1 associates with over 150 interactors to influence multiple biological processes. The molecular mechanisms that underly spatial and temporal regulation of these interactions, which are crucial for proper cell functions, remain poorly understood. The receptor tyrosine kinase MET phosphorylates IQGAP1 on Tyr¹⁵¹⁰. Separately, Src homology 2 (SH2) domains mediate protein-protein interactions by binding specific phosphotyrosine residues. Here, we investigate whether MET-catalyzed phosphorylation of Tyr¹⁵¹⁰ of IQGAP1 regulates the docking of SH2-containing proteins. Using a peptide array, we identified SH2 domains from several proteins, including the non-receptor tyrosine kinases Abl1 and Abl2, that bind to the Tyr¹⁵¹⁰ of IQGAP1 in a ph

SUBMITTER: Thines L 

PROVIDER: S-EPMC9913818 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature