IL18 Receptor Signaling Inhibits Intratumoral CD8⁺ T-Cell Migration in a Murine Pancreatic Cancer Model.

ABSTRACT: In pancreatic ductal adenocarcinoma (PDAC), the infiltration of CD8⁺ cytotoxic T cells (CTLs) is an important factor in determining prognosis. The migration pattern and interaction behavior of intratumoral CTLs are pivotal to tumor rejection. NLRP3-dependent proinflammatory cytokines IL-1β and IL-18 play a prominent role for CTL induction and differentiation. Here, we investigate the effects of T-cellular IL-1R and IL-18R signaling for intratumoral T-cell motility. Murine adenocarcinoma cell line Panc02 was stably transfected with ovalbumin (OVA) and fluorophore H2B-Cerulean to generate PancOVA H2B-Cerulean tumor cells. Dorsal skinfold chambers (DSFC) were installed on wild-type mice, and PancOVA H2B-Cerulean tumor cells were implanted into the chambers. PancOVA spheroids were f

SUBMITTER: Nasiri E 

PROVIDER: S-EPMC9913970 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature