A Combinational Optimization Method for Efficient Production of Indigo by the Recombinant Escherichia coli with Expression of Monooxygenase and Malate Dehydrogenase.

ABSTRACT: Indigo pigment is a widely used pigment, and the use of biosynthesis to ferment indigo has become a hot research topic. Based on previous research, the indigo could be biosynthesized via the styrene oxygenation pathway, which is regulated by intracellular redox-cofactor rebalancing. In this work, the malate dehydrogenase (mdh) gene was selected as an NADH regeneration element to improve the intracellular cofactor regeneration level, and it was co-expressed with the styrene monooxygenase (styAB) gene by pET-28a(+) vector in E. coli for enhancing indigo production. The P_T7 and P_cat promoter was constructed to change the styAB gene and mdh gene from inducible expression to constitutive expression, since the expressing vect

SUBMITTER: Pan Z 

PROVIDER: S-EPMC9914922 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature