A Versatile Toolset for Genetic Manipulation of the Wine Yeast Hanseniaspora uvarum.

ABSTRACT: Hanseniaspora uvarum is an ascomycetous yeast that frequently dominates the population in the first two days of wine fermentations. It contributes to the production of many beneficial as well as detrimental aroma compounds. While the genome sequence of the diploid type strain DSM 2768 has been largely elucidated, transformation by electroporation was only recently achieved. We here provide an elaborate toolset for the genetic manipulation of this yeast. A chromosomal replication origin was isolated and used for the construction of episomal, self-replicating cloning vectors. Moreover, homozygous auxotrophic deletion markers (Huura3, Huhis3, Huleu2, Huade2) have been obtained in the diploid genome as future recipients and a proof of principle for the applic

SUBMITTER: Heinisch JJ 

PROVIDER: S-EPMC9915424 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature