Production of antigenically stable enterovirus A71 virus-like particles in Pichia pastoris as a vaccine candidate.

ABSTRACT: Enterovirus A71 (EVA71) causes widespread disease in young children with occasional fatal consequences. In common with other picornaviruses, both empty capsids (ECs) and infectious virions are produced during the viral lifecycle. While initially antigenically indistinguishable from virions, ECs readily convert to an expanded conformation at moderate temperatures. In the closely related poliovirus, these conformational changes result in loss of antigenic sites required to elicit protective immune responses. Whether this is true for EVA71 remains to be determined and is the subject of this investigation. We previously reported the selection of a thermally resistant EVA71 genogroup B2 population using successive rounds of heating and passage. The mutations found in the structural protein-codi

SUBMITTER: Kingston NJ 

PROVIDER: S-EPMC9915507 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature