Gnotobiotic zebrafish microbiota display inter-individual variability affecting host physiology.

ABSTRACT: Gnotobiotic animal models reconventionalized under controlled laboratory conditions with multi-species bacterial communities are commonly used to study host-microbiota interactions under presumably more reproducible conditions than conventional animals. The usefulness of these models is however limited by inter-animal variability in bacterial colonization and our general lack of understanding of the inter-individual fluctuation and spatio-temporal dynamics of microbiota assemblies at the micron to millimeter scale. Here, we show underreported variability in gnotobiotic models by analyzing differences in gut colonization efficiency, bacterial composition, and host intestinal mucus production between conventional and gnotobiotic zebrafish larvae re-conventionalized with a mix of 9 bacteria i

SUBMITTER: Adade EE 

PROVIDER: S-EPMC9915576 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature