Comparison of commercial DNA extraction kits for whole metagenome sequencing of human oral, vaginal, and rectal microbiome samples.

ABSTRACT:

Introduction

Advancements in DNA extraction and sequencing technologies have been fundamental in deciphering the significance of the microbiome related to human health and pathology. Whole metagenome shotgun sequencing (WMS) is gaining popularity in use compared to its predecessor (i.e., amplicon-based approaches). However, like amplicon-based approaches, WMS is subject to bias from DNA extraction methods that can compromise the integrity of sequencing and subsequent findings. The purpose of this study was to evaluate systematic differences among four commercially available DNA extraction kits frequently used for WMS analysis of the microbiome.

Methods

Oral, vaginal, and rectal swabs were collected in replicates of four by a healthcare provider from five participants and ran

SUBMITTER: Wright ML 

PROVIDER: S-EPMC9915679 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature