Using single-cell RNA sequencing to generate cell-type-specific split-GAL4 reagents throughout development.

ABSTRACT: Cell-type-specific tools facilitate the identification and functional characterization of distinct cell types, which underly the complexity of neuronal circuits. A large collection of existing genetic tools in Drosophila relies on enhancer activity to label different subsets of cells. These enhancer-based GAL4 lines often fail to show a predicable expression pattern to reflect the expression of nearby gene(s), partly due to an incomplete capture of the full gene regulatory elements. While genetic intersectional technique such as the split-GAL4 system further improve cell-type-specificity, it requires significant time and resource to generate and screen through combinations of enhancer expression patterns. In addition, since existing enhancer-based split-GAL4 lines that show cell-type-speci

SUBMITTER: Chen YD 

PROVIDER: S-EPMC9915743 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature