Glycoproteomics in Cerebrospinal Fluid Reveals Brain-Specific Glycosylation Changes.

ABSTRACT: The glycosylation of proteins plays an important role in neurological development and disease. Glycoproteomic studies on cerebrospinal fluid (CSF) are a valuable tool to gain insight into brain glycosylation and its changes in disease. However, it is important to consider that most proteins in CSFs originate from the blood and enter the CSF across the blood-CSF barrier, thus not reflecting the glycosylation status of the brain. Here, we apply a glycoproteomics method to human CSF, focusing on differences between brain- and blood-derived proteins. To facilitate the analysis of the glycan site occupancy, we refrain from glycopeptide enrichment. In healthy individuals, we describe the presence of heterogeneous brain-type N-glycans on prostaglandin H2-D isomerase alongside the dominant plasma-

SUBMITTER: Baerenfaenger M 

PROVIDER: S-EPMC9916115 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature