Simple Affinity-Based Method for Concentrating Viruses from Wastewater Using Engineered Curli Fibers.

ABSTRACT: Wastewater surveillance is a proven method for tracking community spread and prevalence of some infectious viral diseases. A primary concentration step is often used to enrich viral particles from wastewater prior to subsequent viral quantification and/or sequencing. Here, we present a simple procedure for concentrating viruses from wastewater using bacterial biofilm protein nanofibers known as curli fibers. Through simple genetic engineering, we produced curli fibers functionalized with single-domain antibodies (also known as nanobodies) specific for the coat protein of the model virus bacteriophage MS2. Using these modified fibers in a simple spin-down protocol, we demonstrated efficient concentration of MS2 in both phosphate-buffered saline (PBS) and in the wastewater matrix. Additional

SUBMITTER: Birnbaum DP 

PROVIDER: S-EPMC9916486 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature