Degraded RNA from Human Anterior Cruciate Ligaments Yields Valid Gene Expression Profiles.

ABSTRACT: Correlating gene expression patterns with biomechanical properties of connective tissues provides insights into the molecular processes underlying the tissue growth and repair. Cadaveric specimens such as human knees are widely considered suitable for biomechanical studies, but their usefulness for gene expression experiments is potentially limited by the unavoidable, nuclease-mediated degradation of RNA. Here, we tested whether valid gene expression profiles can be obtained using degraded RNA from human anterior cruciate ligaments (ACLs). Human ACL RNA (N = 6) degraded in vitro by limited ribonuclease digestion resemble highly degraded RNA isolated from cadaveric tissue. PCR threshold cycle (C_t) values for 90 transcripts (84 extracellular matrix, 6 housekeeping) in degraded RNA

SUBMITTER: Ashton MN 

PROVIDER: S-EPMC9916516 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature