Redox-Dependent Modulation of Human Liver Progenitor Cell Line Fate.

ABSTRACT: Redox homeostasis is determinant in the modulation of quiescence/self-renewal/differentiation of stem cell lines. The aim of this study consisted of defining the impact of redox modifications on cell fate in a human hepatic progenitor line. To achieve this, the HepaRG cell line, which shows oval ductular bipotent characteristics, was used. The impact of redox status on the balance between self-renewal and differentiation of HepaRG cells was investigated using different methodological approaches. A bioinformatic analysis initially proved that the trans-differentiation of HepaRG toward bipotent progenitors is associated with changes in redox metabolism. We then exposed confluent HepaRG (intermediate differentiation phase) to oxidized (H₂O₂) or reduced (N-acetylcysteine)

SUBMITTER: Bellanti F 

PROVIDER: S-EPMC9916526 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature