Antiproliferative Activity of Antibiotics through DNA Binding Mechanism: Evaluation and Molecular Docking Studies.

ABSTRACT: The antiproliferative activity of three antibiotics clinically use, was studied through DNA inhibition mechanisms, ex vivo, in silico and in vitro. The ex vivo interaction of DNA with ciprofloxacin hydrochloride (CIP·HCl), penicillin G sodium salt (PEN·Na), and tetracycline hydrochloride (TC·HCl) was determined by UV-Vis spectra and viscosity measurements. Furthermore, their binding constants (K_b) toward CT-DNA were calculated (K_b = (2.8 ± 0.6) × 10⁴ (CIP·HCl), (0.4 ± 0.1) × 10⁴ (PEN·Na) and (6.9 ± 0.3) × 10⁴ (TC·HCl) Μ^-1). Docking studies on the binding interactions of antibiotics with DNA were performed to rationalize the ex vivo results. The in vitro antiproliferativ

SUBMITTER: Magklaras AC 

PROVIDER: S-EPMC9916907 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature