Regional Differences in Ca²⁺ Signaling and Transverse-Tubules across Left Atrium from Adult Sheep.

ABSTRACT: Cardiac excitation-contraction coupling can be different between regions of the heart. Little is known at the atria level, specifically in different regions of the left atrium. This is important given the role of cardiac myocytes from the pulmonary vein sleeves, which are responsible for ectopic activity during atrial fibrillation. In this study, we present a new method to isolate atrial cardiac myocytes from four different regions of the left atrium of a large animal model, sheep, highly relevant to humans. Using collagenase/protease we obtained calcium-tolerant atrial cardiac myocytes from the epicardium, endocardium, free wall and pulmonary vein regions. Calcium transients were slower (time to peak and time to decay) in free wall and pulmonary vein myocytes compared to the epicardium an

SUBMITTER: Cros C 

PROVIDER: S-EPMC9916916 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature