Utilizing the ¹H-¹⁵N NMR Methods for the Characterization of Isomeric Human Milk Oligosaccharides.

ABSTRACT: Human milk oligosaccharides (HMOs) are structurally complex unconjugated glycans that are the third largest solid fraction in human milk after lactose and lipids. HMOs are in the forefront of research since they have been proven to possess beneficial health effects, especially on breast-fed neonates. Although HMO research is a trending topic nowadays, readily available analytical methods suitable for the routine investigation of HMOs are still incomplete. NMR spectroscopy provides detailed structural information that can be used to indicate subtle structural differences, particularly for isomeric carbohydrates. Herein, we propose an NMR-based method to identify the major isomeric HMOs containing GlcNAc and/or Neu5Ac building blocks utilizing their amide functionality. Experimental conditio

SUBMITTER: Garadi Z 

PROVIDER: S-EPMC9917050 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature