Construction of Biocatalysts Using the P450 Scaffold for the Synthesis of Indigo from Indole.

ABSTRACT: With the increasing demand for blue dyes, it is of vital importance to develop a green and efficient biocatalyst to produce indigo. This study constructed a hydrogen peroxide-dependent catalytic system for the direct conversion of indole to indigo using P450BM3 with the assistance of dual-functional small molecules (DFSM). The arrangements of amino acids at 78, 87, and 268 positions influenced the catalytic activity. F87G/T268V mutant gave the highest catalytic activity with k_cat of 1402 min^-1 and with a yield of 73%. F87A/T268V mutant was found to produce the indigo product with chemoselectivity as high as 80%. Moreover, F87G/T268A mutant was found to efficiently catalyze indole oxidation with higher activity (k_cat/K_m = 1388 mM

SUBMITTER: Li Y 

PROVIDER: S-EPMC9917246 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature