Advanced Glycation End Products Effects on Adipocyte Niche Stiffness and Cell Signaling.

ABSTRACT: Adipose tissue metabolism under hyperglycemia results in Type II diabetes (T2D). To better understand how the adipocytes function, we used a cell culture that was exposed to glycation by adding intermediate carbonyl products, which caused chemical cross-linking and led to the formation of advanced glycation end products (AGEs). The AGEs increased the cells and their niche stiffness and altered the rheological viscoelastic properties of the cultured cells leading to altered cell signaling. The AGEs formed concomitant with changes in protein structure, quantified by spectroscopy using the 8-ANS and Nile red probes. The AGE effects on adipocyte differentiation were viewed by imaging and evidenced in a reduction in cellular motility and membrane dynamics. Importantly, the alteration led to red

SUBMITTER: Izgilov R 

PROVIDER: S-EPMC9917270 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature