Purification of Functional Gene Transfer Agents Using Two-Step Preparative Monolithic Chromatography.

ABSTRACT:

Background

Gene transfer agents (GTAs) are phage-like particles that transfer cellular genomic DNA between cells. A hurdle faced in studying GTA function and interactions with cells is the difficulty in obtaining pure and functional GTAs from cultures.

Materials and methods

We used a novel two-step method for purification of GTAs from R. capsulatus by monolithic chromatography.

Results

Our efficient and simple process had advantages compared to previous approaches. The purified GTAs retained gene transfer activity and the packaged DNA could be used for further studies.

Conclusions

This method is applicable to GTAs produced by other species and small phages, and could be useful for therapeutic applications.

SUBMITTER: Langille E 

PROVIDER: S-EPMC9917305 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature