Dataset Information

Comparative Genomics of Six Lytic Bacillus subtilis Phages from the Southwest United States.

ABSTRACT:

Background

Despite their importance to microbial dynamics involving Bacillus subtilis, we have a limited understanding of the diversity of phages that can lyse this model organism.

Materials and methods

Phages were isolated from soil samples collected from various sites in the southwest U.S. deserts on a wild B. subtilis strain. Their genomes were assembled, characterized, and bioinformatically compared.

Results

Six Siphoviruses with high nucleotide and amino acid similarity to each other (>80%) but very limited similarity to phages currently in GenBank were isolated. These phages have double-stranded DNA genomes (55,312 to 56,127 bp) with 86-91 putative protein coding genes, and a low GC content. Comparative genomics reveal differences in loci encoding proteins that are putatively involved in bacterial adsorption with evidence for genomic mosaicism and a possible role for small genes.

Conclusions

A comparative approach provides insights into phage evolution, including the role of indels in protein folding.

SUBMITTER: Vill AC

PROVIDER: S-EPMC9917325 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Publications

Comparative Genomics of Six Lytic <i>Bacillus subtilis</i> Phages from the Southwest United States.

Vill Albert C AC Delesalle Véronique A VA Tomko Brianne E BE Lichty Katherine B KB Strine Madison S MS Guffey Alexandra A AA Burton Elizabeth A EA Tanke Natalie T NT Krukonis Greg P GP

PHAGE (New Rochelle, N.Y.) 20220919 3

<h4>Background</h4>Despite their importance to microbial dynamics involving <i>Bacillus subtilis</i>, we have a limited understanding of the diversity of phages that can lyse this model organism.<h4>Materials and methods</h4>Phages were isolated from soil samples collected from various sites in the southwest U.S. deserts on a wild <i>B. subtilis</i> strain. Their genomes were assembled, characterized, and bioinformatically compared.<h4>Results</h4>Six Siphoviruses with high nucleotide and amino ...[more]

PMID: 36793550

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