Comparative Genomics of Six Lytic Bacillus subtilis Phages from the Southwest United States.

ABSTRACT:

Background

Despite their importance to microbial dynamics involving Bacillus subtilis, we have a limited understanding of the diversity of phages that can lyse this model organism.

Materials and methods

Phages were isolated from soil samples collected from various sites in the southwest U.S. deserts on a wild B. subtilis strain. Their genomes were assembled, characterized, and bioinformatically compared.

Results

Six Siphoviruses with high nucleotide and amino acid similarity to each other (>80%) but very limited similarity to phages currently in GenBank were isolated. These phages have double-stranded DNA genomes (55,312 to 56,127 bp) with 86-91 putative protein coding genes, and a low GC content. Comparative genomics reveal differences in loci encoding

SUBMITTER: Vill AC 

PROVIDER: S-EPMC9917325 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature