Scalable Combinatorial Assembly of Synthetic DNA for Tracking Applications.

ABSTRACT: Synthetic DNA barcodes are double-stranded DNA molecules designed to carry recoverable information, information that can be used to represent and track objects and organisms. DNA barcodes offer robust, sensitive detection using standard amplification and sequencing techniques. While numerous research groups have promoted DNA as an information storage medium, less attention has been devoted to the design of economical, scalable DNA barcode libraries. Here, we present an alternative modular approach to sequence design. Barcode sequences were constructed from smaller, interchangeable blocks, allowing for the combinatorial assembly of numerous distinct tags. We demonstrated the design and construction of first-generation (N = 256) and second-generation (N = 512) modular barcode libraries, from

SUBMITTER: Stuart JD 

PROVIDER: S-EPMC9917336 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature