Ontology highlight
ABSTRACT:
SUBMITTER: Gyongy Z
PROVIDER: S-EPMC9917445 | biostudies-literature | 2023 Feb
REPOSITORIES: biostudies-literature
Gyöngy Zsuzsanna Z Mocsár Gábor G Hegedűs Éva É Stockner Thomas T Ritter Zsuzsanna Z Homolya László L Schamberger Anita A Orbán Tamás I TI Remenyik Judit J Szakacs Gergely G Goda Katalin K
eLife 20230210
ABCG2 is an exporter-type ABC protein that can expel numerous chemically unrelated xeno- and endobiotics from cells. When expressed in tumor cells or tumor stem cells, ABCG2 confers multidrug resistance, contributing to the failure of chemotherapy. Molecular details orchestrating substrate translocation and ATP hydrolysis remain elusive. Here, we present methods to concomitantly investigate substrate and nucleotide binding by ABCG2 in cells. Using the conformation-sensitive antibody 5D3, we show ...[more]