Optimization of the Hemolysis Assay for the Assessment of Cytotoxicity.

ABSTRACT: In vitro determination of hemolytic properties is a common and important method for preliminary evaluation of cytotoxicity of chemicals, drugs, or any blood-contacting medical device or material. The method itself is relatively straightforward, however, protocols used in the literature vary substantially. This leads to significant difficulties both in interpreting and in comparing the obtained values. Here, we examine how the different variables used under different experimental setups may affect the outcome of this assay. We find that certain key parameters affect the hemolysis measurements in a critical manner. The hemolytic effect of compounds tested here varied up to fourfold depending on the species of the blood source. The use of different types of detergents used for generating posi

SUBMITTER: Sæbo IP 

PROVIDER: S-EPMC9917735 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature