MiR-197-3p Promotes Osteosarcoma Stemness and Chemoresistance by Inhibiting SPOPL.

ABSTRACT: First-line treatment for osteosarcoma includes chemotherapy and surgery. However, the five-year survival rate of refractory osteosarcoma remains unsatisfactory. Osteosarcoma cancer stem cells, possessing stemness and chemoresistance, are one of the critical causes of poor response to chemotherapy. Elucidating regulatory signaling pathways of osteosarcoma cancer stem cells may provide a rationale for improving regimens against chemoresistant osteosarcoma. Methotrexate (MTX)-resistant osteosarcoma cells were established. microRNA expression profiles were used for detecting differentially expressed microRNA in resistant clones and the parental cells. microRNA target databases were employed to predict potential microRNA and mRNA interactions. Flow cytometry was performed to measure stem cell m

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9917813 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature