Role of the Water-Metal Ion Bridge in Quinolone Interactions with Escherichia coli Gyrase.

ABSTRACT: Fluoroquinolones are an important class of antibacterials, and rising levels of resistance threaten their clinical efficacy. Gaining a more full understanding of their mechanism of action against their target enzymes-the bacterial type II topoisomerases gyrase and topoisomerase IV-may allow us to rationally design quinolone-based drugs that overcome resistance. As a step toward this goal, we investigated whether the water-metal ion bridge that has been found to mediate the major point of interaction between Escherichia coli topoisomerase IV and Bacillus anthracis topoisomerase IV and gyrase, as well as Mycobacterium tuberculosis gyrase, exists in E. coli gyrase. This is the first investigation of the water-metal ion bridge and its function in a Gram-negative gyr

SUBMITTER: Carter HE 

PROVIDER: S-EPMC9917921 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature