Trace detection of SARS-CoV-2 N-protein by diamond solution-gate field-effect transistor.

ABSTRACT: In this study, we introduced H-terminated diamond solution-gate field-effect transistor (H-diamond SGFET) to detect trace SARS-CoV-2 N-protein, which plays an important role in replication and transcription of viral RNA. 1-Pyrenebutyric acid-N-hydroxy succinimide ester (Pyr-NHS) was modified on H-diamond surface as linker, on which the specific antibody of SARS-CoV-2 N-protein was catenated. Fourier transform infrared spectrum, scanning electron microscope and energy dispersive spectrum were utilized to demonstrate the modification of H-diamond with Pyr-NHS and antibody. Shifts of I_DS(max) at V_GS = -500 mV in transfer characteristics of H-diamond SGFET was observed to determine N-protein concentration in phosphate buffer solution. Good linear relationship between I

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC9918317 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature